柠檬酸钠促进S-腺苷蛋氨酸和谷胱甘肽联合高产

Increased Co-production of S-adenosylmethionine and Glutathione by Sodium Citrate Addition

考察了柠檬酸钠对S-腺苷蛋氨酸(SAM)和谷胱甘肽(GSH)联产发酵的影响,发现柠檬酸钠有利于SAM和GSH的联合高产。采用响应面分析法对柠檬酸钠浓度及其添加时间进行优化,模型预测和验证实验结果均表明在联产发酵6 h时一次性添加10 g/L柠檬酸钠的效果最佳。通过对SAM和GSH联产发酵过程进行分析,发现柠檬酸钠能够显著提高胞内NADH和ATP的水平,为SAM和GSH的过量合成提供了足够的能量物质,也为类似耗能化合物的生物合成及其发酵高产提供了可行的优化策略。

S-adenosylmethionine （SAM） and glutathione （GSH） are both important small S-contained compounds in cells. The effects of sodium citrate Candida utilis CCTCC M 209298 were investigated on the fermentative co-production of SAM and GSH with in flasks. Sodium citrate was found to be beneficial for the high co-production of SAM and GSH. The response surface analysis was applied in the optimization of sodium citrate concentration and addition time, and a strategy of 10 g/L sodium citrate addition at 6 h was predicted by a statistical model and verified to be the best approach for increased co-production of SAM and GSH. Based on the results derived from the kinetic analysis on the batch fermentation processes, intracellular levels of NADH and ATP could be significantly improved by sodium citrate, and which in turn provided essential energy substance needed for the over-production of SAM and GSH. The results also provide a potential approach for efficient production of analogical useful chemicals biosynthesized with the consumption of energy.