响应面法优化产蛋白酶海洋细菌的培养条件

Optimization of Cultivation Conditions for Protease Production from Marine Bacteria by Response Surface Methodology

借助Minitab 16数据处理软件对海洋细菌SE2011的产酶条件进行优化。在单因素试验的基础上,利用Plackett-Burman设计从8个影响因素中筛选出3主要影响因素(P<0.05),即NaCl、培养基初始pH和装液量。在此基础上,用最陡爬坡试验逼近最大响应区域,再利用Box-Behnken试验设计及响应面分析法进行回归分析,通过求解回归方程得到产酶的最优条件为:NaCl 2.0%,培养基初始pH 8.0,装液量23ml。经过优化,蛋白酶活力达到1336.462 U/ml,较初始酶活力(733.269 U/ml)提高了82.3%。

Minitab 16 software was applied to optimize the conditions for protease production of marine bacteria SE2011. On the basis of single factor experiments, the Plackett-Burman design was utilized to analyze effect factors. Among the eight factors studied, salinity, pH value and volume of medium per flask had significant effects on protease production （ P 〈 0.05 ）. The path of steepest ascent was undertaken to approach the optimal region of the protease production. The regression analysis was further investigated by using Box-Behnken design and response surface analysis. By solving regession equation, the highest protease production was obtained at salinity 2.0%, pH 8.0 and volume of medium 23 ml per flask. The protease production reached to 1336. 462 U/ml, which increased 82.3% compared to the initial 733. 269 U/ml.