高吸水性树脂联合超声造影剂对HIFU杀伤棘球蚴原头节的增效作用

Enhancement of in Vitro Protoscolicidal Effects of High-intensity Focused Ultrasound by a Superabsorbent Polymer and Ultrasound Contrast Agent

为探索高吸水性树脂（superabsorbentpolymer，SAP）与超声造影剂（ultrasoundcontrastagent，UCA）协同增强高强度聚焦超声（highintensityfocusedultrasound，HIFU）杀伤棘球蚴原头节的效果，将30管棘球蚴原头节悬液（各含约6000--7500个原头节）分为5组，分别为A组（对照组，仅给予无功率HIFU普通超声辐照）、B组（单纯HIFU辐照，50W）、C组（HIFU辐照＋10txlUCA）、D组（HIFU辐照＋0．01gSAP）和E组（HIFU辐照＋10μlUCA＋0．01gSAP）。结果显示，B组超声图像灰度变化明显，悬液温度和原头节死亡率（26．0℃±012℃、30．4％）均比A组的（18．0℃±0．1℃、1．9％）高（P〈0．01）。C、D组的超声图像灰度变化比B组更为明显，悬液温度（27．0℃±0．2℃，28．2℃±0．2℃）和原头节死亡率（49．96％，53．69％）也比B组的高（P〈0．01）。E组悬液温度（28．4℃±0．3℃）与C、D组的差异无统计学意义（P〉0．05），但原头节死亡率（69．7％）远高于C、D组（P〈0．01），且死亡的原头节结构破坏较其余3组更为严重，内部结构消失。

This study evaluated whether or not a superabsorbent polymer（SAP） combined with ultrasound contrast agent （UCA） could enhance damage efficacy of high intensity focused ultrasound （HIFU） on Echinococcus granulosus protoscoleces in vitro. Thirty test tubes each with 6 000-7 500 protoscolices were divided into 5 groups： group A （blank control） without HIFU treatment, group B treated with HIFU （50 W） only, group C treated with 10 jxl UCA and HIFU, group D treated with 0.01 g SAP and HIFU, group E treated with 10 μl UCA, 0.01g SAP, and HIFU. In group B, echo enhancement of ultrasound image, suspension temperature（26.0 ℃±0.2 ℃） and protoscoleces mortality（30.4%） were higher than that of group A（18.0 ℃±0.1℃, 1.9%）（P〈0.01 ）. Compared with group B, the echo enhancement of ultrasound image, suspension temperature （27.0 ℃±0.2 ℃, 28.2 ℃±0.2 ℃） and protoscoleces mortality （50.0%, 53.7%） of groups C and D increased significantly （P〈0.01）. In group E, more protoseoleees were stained in red and their internal structures were indistinct. By chi-square test, the protoscoleces mortality of group E（69.7%） was higher than that of groups C and D （P〈 0.01）. There was no sinificant difference in suspension temperature among the 3 groups.