荔枝水孔蛋白基因LcPIP的克隆与组织特异性表达研究

Cloning and Tissue Specificity Expression of the Aquaporin Genes from Litchi

从荔枝组织中克隆了9个质膜水孔蛋白基因(LcPIP)的cDNA全长序列,并研究了其组织特异性表达。结果表明:9个LcPIP分为PIP1和PIP2两类。定量PCR结果表明9个LcPIPs在荔枝的不同组织中均表达,其中LcPIP1-2、LcPIP1-4、LcPIP2-1、LcPIP2-2在花中的表达量相对较高,LcPIP1-1在茎中相对较高,LcPIP1-2在果肉中较高,LcPIP2-5在种子中的表达量仅次于果皮,而LcPIP1-3在叶中最高,LcPIP2-4在根中最高。LcPIP1-1、LcPIP2-4、LcPIP2-5在果皮中表达量较高,而LcPIP2-3在果皮中特异表达,且在所有成员中表达量最高,可能与果皮组织水分运输有关。

Nine plasma membrane aquaporin genes named LcPIP were cloned from litchi and their tissue specificity expressions were studied in order to provide the information for further research on litchi preservation during the postharvest storage. These nine LcPIP genes can be divided into two groups,PIP1 and PIP2. The real-time RT-PCR result showed that LcPIP1-2,LcPIP1-4,LcPIP2-1 and LcPIP2-2 had higher expression level in the flower than other tissues. LcPIP1-1,LcPIP1-2,LcPIP1-3,LcPIP2-4, LcPIP2-5 had higher expression level in stem,pulp,leaf,root and pericarp,respectively. All the expressions of LcPIP1-1,LcPIP2-4 and LcPIP2-5 were higher in pericarp than most of other tissues, which indicated that they may be involved with water transportation of litchi pericarp. LcPIP2-3 was expressed specifically in the pericarp and has a higher expression than any other member.