摘要
对21个枇杷属材料和1个枇杷近缘属材料(石斑木)用去壁低渗—火焰干燥法进行染色体制片,进行核型分析,结果发现:所试材料的体细胞染色体数均为2n=34,为二倍体,染色体类型包括中部(m)、近中部(sm)和近端部(st)着丝点染色体(st,仅存在于大花枇杷中)3种,核型公式为2n=2x=34=16~24m+10~18sm(+2st),属2A对称型核型。根据各个种的中部着丝点染色体(m)的多少,可将22个材料初步分为5类,从第1类到第5类枇杷,核型不对称性依次增强,表明其系统演化地位逐渐进化。
In this paper the wall degradation hypotonic method was used to prepare mitotic chromosome sample of 21 materials(16 species,4 varieties,1 interspecific hybrid)of Eriobotrya and 1 closed genus of Eriobotrya[Rhaphiolepis indica(L.)Lindl.]. The results indicated that the chromosome number was 2n = 2x = 34 in all the materials,and the karyotypes of Eriobotrya and Rhaphiolepis indica consist of three kinds of chromosome:m,sm,and s(tonly in E. cavaleriei Rehd). The karyotype formula was 2n = 2x = 34 =(16 to 24)m +(10 to 18)sm(+ 2st)for all materials. According to the karyotype data, it can be concluded that the karyotypes of 21 Eriobotrya materials belongs to Stebbins’2A,which is a symmetrical karyotype. According to the karyotype formula we preliminarily classified these Eriobotrya materials into 5 groups. The asymmetry increased gradually from the first group to the fifth group,which indicated the systematic evolution among groups is more and more advanced.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第8期1465-1474,共10页
Acta Horticulturae Sinica
基金
公益性行业科研专项(201003073)
亚热带农业生物资源保护与利用国家重点实验室项目
广东普通高校重点实验室华南园艺作物种质资源创新与利用项目(KLB11008)
关键词
枇杷属
核型分析
系统演化
Eriobotrya
karyotype analysis
evolution