摘要
以无性系茶树品种‘龙井43’花粉为材料,研究了低温对茶树花粉萌发和花粉管伸长的影响及其与一氧化氮(NO)的关系。结果显示低温显著抑制了茶树花粉萌发和花粉管伸长,且表现出明显的时间依赖性;外源NO供体硝普钠(SNP)产生类似的抑制效应,并表现出明显的浓度依赖性,其抑制效应被NO清除剂(cPTIO)和一氧化氮合酶(NOS)抑制剂(L-NNA)所缓解。此外,低温处理促进茶树花粉管NO释放并伴随着NOS活性的提高。上述研究结果表明,低温抑制茶树花粉萌发和花粉管伸长与其诱导NO释放有关,且低温诱导的NO释放部分依赖于NOS催化产生。
The pollen germination and pollen tube growth of tea plant,as well as their relationship with nitric oxide（NO）under low-temperature were studied using pollen of culivar‘Longjing 43’. The results showed that low-temperature significantly inhibited the tea pollen germination and pollen tube growth in a time-dependent manner. Similar inhibitory effects in a dose-dependent manner were observed with exogenous NO donor（Sodium Nitroprusside,SNP）. Both NO scavenger（cPTIO）and nitric oxide synthase（NOS）inhibito（rL-NNA）partially alleviated this inhibitory effect. Furthermore,the NO releasing along with the NOS activity were promoted in tea pollen tubes under low-temperature. The results indicated that the inhibitory effects of low-temperature treatment on tea pollen germination and pollen tube growth are related to NO generating from NOS activity.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第8期1535-1540,共6页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31000315
30972403
30800884)
现代农业产业技术体系建设专项资金项目(CARS-23)
江苏省企业博士后集聚计划项目(2010)
中国博士后基金项目(20100481158)
林木遗传与生物技术省部共建重点实验室(南京林业大学)开放课题(FGB200904)
教育部博士点新教师基金项目(20090097120049)
江苏省科技支撑计划资金项目(BE2011319
BE2012440
BE2012441)
关键词
茶树
低温
花粉萌发
花粉管伸长
一氧化氮
tea plant
low-temperature
pollen germination
pollen tube growth
nitric oxide
