雄激素受体基因在绵羊公羔不同组织中的表达特性及性腺中的定位

The Expression of Androgen Receptor Gene in Different Tissues and Cellular Localization in Gonad of Male Sheep Lambs

旨在研究雄激素受体(Androgen receptors,ARs)基因在绵羊公羔不同组织中的表达特性及在性腺中的定位。本研究以QRT-PCR法检测各组织ARmRNA表达规律,免疫组化技术对睾丸和附睾中AR进行定位分析,蛋白质印迹技术对AR蛋白表达量进行定性研究。结果显示:(1)AR在不同组织中均有不同程度的表达,其中在睾丸和心脏中大量表达;(2)在附睾头、体、尾部单层柱状上皮细胞和间质细胞中检测到较弱的阳性信号;睾丸组织间质细胞、精原细胞、管周肌样细胞检测到强阳性信号。综上表明:AR在绵羊不同组织中广泛表达,AR在性腺的发育和精子的成熟过程以及对心脏的保护方面发挥重要作用,其作用机制需进一步研究。

The objectives of this study were to evaluate the expression of Androgen receptors（AR） in various tissues and the cellular localization of gonad of the male sheep lambs.In this experiment,mRNA expression was detected by real-time fluorescent quantitative PCR.Cellular localization of AR in testis and epididymidis were examined by immunohistochemistry.Qualitative investigation of AR protein was expressed by Western blotting.The result showed that：（1） Sheep AR mRNA was expressed in all the tissues,and abundantly expressed in testis and heart.（2） Weak positive signals were detected in pseudo stratified ciliated columnar epithelium and interstitial tissue of EpH,EpB and EpT.Strong positive signals were detected in interstitial tissue,spermatogenous cells and myoid cells of sheep testis.Sheep AR was widely expressed in all the tissues.AR plays an important role in gonadal developing and sperm maturation processing and heart protecting,the mechanisms need further research.