妊娠期多溴联苯醚暴露对雄性仔鼠性发育的影响

Effect of maternal BDE-209 exposure on sexual development in male offspring rats

目的观察妊娠期多溴联苯醚（BDE209）暴露对雄性仔鼠性发育的影响。方法将24只SD妊娠大鼠随机分为3个BDE209染毒组（分别给予100、300和900mg／kgBDE209，于妊娠12～18d进行灌胃染毒）和1个对照组（玉米油）。于出生第4、10、16、21天测定雄性仔鼠体重和身长。各组随机选取12只雄性仔鼠于出生后第21天测定雄性仔鼠肛门-生殖器间距，测定血清睾酮，计算睾丸脏器系数。结果各染毒组雄性仔鼠出生体重、身长与对照组比较，有下降趋势，其中900mg／kg染毒组出生后各观察时间点的体重与身长均明显低于对照组，差异有统计学意义（P〈0．01）。出生后第21天，100、300和900mg／kgBDE209染毒组雄性仔鼠的肛门-生殖器间距分别为（17．82±2．35）、（16．32±1．66）和（15．80±1．34）mm，表现为随染毒剂量的增加呈现下降的趋势，差异有统计学意义（P〈0．05），但与对照组（16．64±2．38mm）比较，差异无统计学意义（Jp〉0．05）。与对照组相比，各染毒组血清睾酮水平、睾丸脏器系数、附睾脏器系数无明显改变，差异无统计学意义（P〉0．05）。结论妊娠期BDE209暴露对雄性仔鼠生长发育有一定影响，但对雄性仔鼠的性发育影响不明显。

Objective To investigate the effect of maternal decabromodiphenyl ether （BDE-209） exposure on the sexual development in male offspring rats. Methods Twenty-four pregnant Sprague-Dawley rats were randomly divided into three BDE-209 exposure groups and one control group. The three BDE-209 exposure groups were given BDE-209 （100, 300, and 900 mg/kg） by gavage on gestational days 12-18, and the control group was given corn oil. The body weight and body length of each newborn male rat was measured at postnatal days 4, 10, 16, and 21. Twelve newborn male rats were randomly selected from each group; anogenital distance was measured at postnatal day 21, serum testosterone was measured, and the organ coefficient of testis was calculated. Results The newborn male rats in all exposure groups showed declining trends in body weight and body length compared with those in the control group, and the 900 mg/kg BDE-209 exposure group had significantly lower body weight and body length than the control group at postnatal days 4, 10, 16, and 21 （P〈0.01）. At postnatal day 21, the 100, 300, and 900 mg/kg BDE-209 exposure groups had anogenital distances of 17.82± 2.35 mm, 16.32±1.66 mm, and 15.80 ±1.34 ram, respectively, demonstrating a significant decrease with increased exposure dose （P〈0.05）, but no significant difference was found when comparing these values with that of the control group （16.64±2.38 mm） （P〉0.05）. There were no significant differences in serum testosterone and organ coefficients of testis and epididymis between the control group and BDE-209 exposure groups （P〉0.05）. Conclusion Maternal exposure to BDE-209 has adverse effect on the growth of male offspring rats, but it leads to no significant changes in sexual development.