2，5-己二酮与人血清体外培养观察吡咯加合物形成

Formation of pyrrole adducts in 2, 5-hexanedione-containing human serum cultured in vitro

目的探讨吡咯加合物的生成与2，5-己二酮（2，5-hexanedione，2，5-HD）浓度之间的关系，为正己烷毒性研究提供实验依据。方法混合正常人离体血清，过滤除菌后配制2，5-HD终浓度为200、100、50、25、10mg／L及空白血清，37℃培养，不同时间点取血清，比色法测定血清中吡咯加合物的生成量，气相色谱法测定血清中2，5-HD剩余量。结果血清中吡咯加合物的含量随培养时间的延长而上升，且与2，5-HD存在剂量依赖关系，实验结束时吡咯加合物的含量在各浓度组间的差异有统计学意义（P〈0．05）；血清中2，5-HD的含量随培养时间的延长而降低，实验结束时按2，5-HD浓度由高到低，2，5-HD浓度分别下降29％、55％、22％、44％和40％；2，5-HD含量的减少量与吡咯加合物的增加量之间存在正相关，按2，5-HD浓度由高到低，相关系数r分别为0．865、0．697、0．835、0．823和0．814。结论随2，5-HD浓度增加，吡咯加合物生成量增加；血清中2，5-HD减少量与吡咯加合物的增加量呈正相关。

Objective To investigate the relationship between formation of pyrrole adducts and concentration of 2, 5-hexanedione （2, 5-HD） and to provide an experimental basis for the study on toxicity of n- hexane. Methods Serum samples were collected from normal persons and were then filtered and sterilized. They were mixed with 2,5-HD to obtain sera with final 2, 5-HD concentrations of 10, 25, 50, 100, and 200 mg/L, and blank serum was also prepared. The sera were cultured at 37℃ and taken at different time points. Colorimetry was used to quantify the pyrrole adducts formed in sera, and gas chromatography was used to measure the remaining 2, 5-HD levels in sera. Results The content of pyrrole adducts increased as the culture proceeded and was dependent on the dose of 2, 5-HD; at the end of the experiment, the content of pyrrole adducts differed significantly across all concentration groups （P〈0.5）. The concentrations of 2,5-HD decreased as the culture proceeded; at the end of the experiment, the concentrations of 2, 5-HD, from the highest to the lowest, decreased by 29%, 55%, 22%, 44%, and 40%, respectively. The decrease in 2, 5-HD had a positive correlation with the increase in pyrrole adducts, and the correlation coefficients for 200-10 mg/L 2, 5-HD were 0.865, 0.697, 0.835, 0.823, and 0.814, respectively. Conclusion The content of formed pyrrole adducts increases as the concentration of 2,5-HD rises; there is a positive correlation between the decrease in 2, 5-HD and the increase in pyrrole addncts in human serum.