摘要
目的:探讨海龙蛤蚧口服液中淫羊藿苷的含量测定方法,为其质量控制提供实验依据。方法:采用高效液相法测定淫羊藿苷的含量,Diamonsil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(25:75),流速为1.0 mL/min,最大检测波长为270 nm。结果:淫羊藿苷在0.023 2~0.740 0μg范围内具有良好的线性关系,回归方程为y=74 988x+22 920,R2=1.0,依测定结果,暂定制剂中淫羊藿苷含量为每毫升不低于1.35μg。结论:该方法简便、准确,灵敏度高,重现性好,可作为海龙蛤蚧口服液质量控制的有效方法。
Objective: To provide experimental evidence for quality control of HaiLong Gejie oral liquid through discussing the content determination method of icariin.Method: High performance liquid chromatography(HPLC) was used for the content determination of icariin,the column was Diamonsil C18(250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-water(25:75),the flow rate was 1.0 mL/min,the detection wavelength was 270nm.Result: Icariin showed the better linear relationship in the range of 0.023 2~0.74 00 μg,and the regression equation was y=74988x+22920,R2=1.0.Based on the test results,the contents of icariin in the tentative preparations were not less than 1.35μg/mL.Conclusion: The method,simple,accurate,sensitive and reproducible,is effective for quality control of HaiLong Gejie oral liquid.
出处
《西部中医药》
2013年第8期22-24,共3页
Western Journal of Traditional Chinese Medicine