摘要
目的:观察藏药镰形棘豆总黄酮苷元对小鼠的急性毒性作用,以评价该药物的安全性。方法:以最大给药浓度和最大剂量(0.1 g/kg)灌胃给药,连续观察7天,判断半数致死剂量的可能性,并据此结果,将昆明种小鼠60只分为3组:实验Ⅰ组(0.2 g/kg)、实验Ⅱ组(0.3 g/kg)和空白对照组(0.5%CMC-Na),连续灌胃给药7天,观察小鼠毒副反应情况,测定出最大耐受量。结果:小鼠口服镰形棘豆总黄酮苷元无法测出LD50,小鼠最大耐受量为300g总黄酮苷元/kg(体质量),相当于60kg(体质量)成人临床生药日用量的378倍。结论:镰形棘豆总黄酮苷元急性毒性较小,具有一定的使用安全性。
Objective: To assess the safety of LianXing JiDou(Oxytropis Falcata Bunge) through surveying acute toxicity test of total flavonoid aglycones from the drug.Method: The mice received intragastric administration in maximum drug concentration and maximum dose for seven consecutive days,the possibility of lethal median dose(LD-50) was judged.Based on the results,sixty mice were divided into three groups: sample test group I(0.2 g/kg),the test group II(0.3 g/kg) and the control group(0.5% CMC-Na),intragastric administration for seven consecutive days,adverse reactions of the mice were observed to determine maximum tolerance dose.Result: All the mice survived and LD50 could not be measured,maximum tolerance dose of the mice was 300g of total flavonoid aglycones per kilogram,equivalent to 378 times of clinical dose of a man weighing 60 kg.Conclusion: Acute toxicity test shows that total flavonoid aglycones from LianXing JiDou is safe to a certain extent.
出处
《西部中医药》
2013年第8期50-52,共3页
Western Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(编号81260633)
甘肃省中医药科技项目(编号2006-298-30)
关键词
镰形棘豆
总黄酮苷元
急性毒性
半数致死量
最大耐受量
LianXing JiDou(Oxytropis Falcata Bunge)
total flavonoid aglycones
acute toxicity
lethal median dose(LD-50)
maximum tolerance dose