猫晶状体超声乳化摘除术后角膜内皮损伤模型的建立及观察

The establishment and observation of an animal model of corneal endothelial injury in cats with operation of phacoemulsification and in traocular lens implantation

目的建立猫晶状体超声乳化摘除人工晶状体植入术后角膜内皮损伤模型。方法家猫10只(20只眼)行双眼晶状体超声乳化摘除人工晶状体植入术,术后第1、3、7、14天裂隙灯下检查术眼眼前节情况;术前、术后14天检查术眼中央角膜厚度及中央角膜内皮细胞密度。结果 20只眼顺利行双眼晶状体超声乳化摘除人工晶状体植入术,术前中央角膜厚度为516.9±18.34μm,术后14天中央角膜厚度为702.1±17.85μm,手术前后相比差异有统计学意义(P<0.05);术前角膜内皮计数为2104.3±156.22个/mm2,术后14天角膜内皮计数为1011.5±153.37个/mm2,手术前后相比差异有统计学意义(P<0.05)。结论成功构建猫晶状体超声乳化摘除人工晶状体植入术后角膜内皮损伤模型,为下一步术后药物应用对角膜内皮损伤修复作用评价提供基础。

Objective To establish an animal model of corneal endothelial injury in eats with operation of phaeoemulsification and intraocular lens implantation （IOL）, in preparation for the next treatment. Methods The operation of phacoemulsification and IOL implantation was performed in 20 eyes of 10 home cats. The operative eyes were examined by slitlamp at 1 day, 3 days, 7 days and 2 weeks after opertation. The central corneal thickness and density of central corneal endothelial cells were examined before and after operation. Results The mean central corneal thickness of the 20 eyes was 516.9±18.34μm before operation, and it was 702.1±17.85μm at 2 weeks after operation. The mean density of central corneal en- dothelial cells of the 20 eyes was 2104.3±156.22 cells/mm^2 before operation, and it was 1011.5±153.37个/mm at 2 weeks after operation. There were significant differences in the central corneal thickness and the density of central cornea/ endothelial cells before and after operation （P〈0.05）. Conclusion The animal model of corneal endothelial injury with operation of phacoemulsifieation and intraocular lens implantation is successfully established in cats.