大鼠心脏移植淋巴细胞多基因差异表达及淋巴细胞表面蛋白分子的表达时相比较

Comparison between lymphocyte multi-gene differential expression and lymphocyte surface protein molecule expression phase in rats undergoing heart transplantation

目的建立大鼠异体异位心脏移植动物模型,观察心脏移植前后淋巴细胞多基因表达和CD4、CD8分子表达及环孢素A对其影响。方法 SD大鼠为供体,Wistar大鼠为受体,分为心脏移植对照组和环孢素A干预两组。移植前、移植后24 h、3 d、7 d、10 d和12 d测定外周血淋巴细胞CD4、CD8分子表达、荧光差异显示分析淋巴细胞编码基因表达水平。结果 (1)移植组:7个基因差异表达,比同组的CD4和CD8分子开始正调表达的时间提前24 h;(2)环孢素A组外周血淋巴细胞的基因表达水平发生变化的有5个基因差异表达;(3)环孢素A组2.3-bisphosphoglycerate比对照组的第一个正调表达时相至少晚24 h,环孢素A还可抑制心脏移植后7 d内外周血淋巴细胞对gig18基因的转录;移植后24 h内,环孢素A组外周血淋巴细胞Na-通道蛋白基因正调表达。结论淋巴细胞多基因差异表达及与表面蛋白分子表达时相比较对于深入研究移植排斥反应机制和环孢素A的作用机制具有一定的意义。

Objective To establish the animal models of variant heterotopic cardiac transplantation in rats in order to observe the effects of lymphocyte multi-gene expression and CD4,CD8 molecule expression as well as cyclosporin A before and after heart transplantation .Methods SD rats were used as donor and Wistar rats were used as recipient .The rats were divided into two groups, transplantation control group and cyclosporine A intervention group.The CD 4, CD8 molecule expression levels in peripheral blood lymphocyte were detected respectively at 24h, 72h,7d,10d, 12d before and after heart transplantation ,and the expression levels of lymphocyte coding gene were analyzed by fluorescence differential display .Results （ 1 ） Tansplantation control group： there were 7 genes differential expression , which were 24h earlier than CD4, CD8 molecular positive regulation expression.（2） Cyclosporine A intervention group：there were 5 genes differential expression in peripheral blood lymphocyte.（3） Cyclosporine A intervention group： 2.3-bisphosphoglycerate positive regulation expression was 24h later than that of control group,fuethermore,cyclosporine A could also inhibit gig18 gene transcription of peripheral blood lymphocyte within 7d after heart transplantation.Within 24h after heart transplantation,sodium channel protein gene was expressed positively in peripheral blood lymphocyte of cyclosporine A intervention group . Conclusion The comparison between lymphocyte multi-gene differential expression and lymphocyte surface protein molecule expression phase plays an important role in researching deeply the transplant rejection reaction mechanism and the action mechanism of cyclosporine A .