上海河流沉积物的有机提取物对3种细胞的毒性效应

Comprehensive Toxicity Efects on Three Cells of Sediment Organic Extractions from Major Rivers in Shanghai

河流沉积物是众多有机污染物的最终储存库.采用索氏抽提法提取出黄浦江及支流苏州河沉积物中的有机污染物,用CCK-8法检测沉积物中的有机提取物对正常人肝细胞L02、人肝癌细胞HepG2以及人乳腺癌细胞MCF-7的细胞增殖毒性,综合评价沉积物中有机污染物的综合毒性效应,进而比较沉积物有机提取物对这3种细胞增殖毒性的差异及两条河流沉积物受有机污染物污染的情况差异.结果表明,黄浦江与苏州河沉积物有机提取物对MCF-7细胞和L02及HepG2细胞表现出不同的毒性效应,黄浦江与苏州河沉积物中的大部分样品对L02和HepG2表现出一定的促增殖效应或没有影响;对MCF-7细胞,除个别样品外,大部分样品表现出细胞增殖毒性效应,并且呈剂量效应及时间效应依赖关系,其毒性大小与采样的位置存在很大关联,这说明黄浦江及苏州河沉积物有机提取物一定程度上受到了有机污染物的污染.另外,两条河流对L02和HepG2的促增殖效应和对MCF-7细胞增殖的毒性效应相当,说明两条河流受有机污染物的污染强度接近及污染物类型相似.

Sediments are considered a repository of many organic pollutants. In this work, organic pollutants are extracted in the sediments from Huangpu River and Suzhou Creek＂ in Shanghai using Soxhlet. The comprehensive toxicity of the sediment organic extractions to three cell proliferations including normal liver cells L02, hepatoma cells HepG2 and human breast cancer cells MCF-7 cells using the CCK-8 assay is evaluated. Proliferation toxicity differences of the sediment organic extracts on the three cells were compared. The results showed that the sediment organic extractions of Huangpu River and Suzhou Creek exhibited different toxic ef- fects on the three cells. For L02 and HepG2, most samples showed positive cell proliferation effect or no effect; while for MCF-7 cells, majority of the samples have different degree toxic effects on MCF-7 cell proliferation and presented dose-effect dependent and time-effect dependent relationships. Besides, the toxicity effect for MCF-7 cell proliferation had a great relevance with the location of sampling. That Huangpu River and Suzhou Creek sediments were polluted by some organic toxicity pollutants can be concluded. In addition, sediments from the two rivers showed a similar toxic effect on the proliferation of L02, HepG2 and MCF-7 cells, indicating that pollution intensity and types of organic pollutants in the two rivers are similar.