大气颗粒物对A549和HUVEC的DNA损伤机制

Genotoxicity of Size-Fractionated Ambient Particulate Matter in A549 and HUVEC Cell Lines

采集北京市海淀区大气颗粒物粗颗粒(PM10 2.5)、细颗粒(PM2.5 0.1)和超细颗粒(PM0.1),分析颗粒物对人肺上皮细胞(human lung epithelial cell,A549)和人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)的基因毒性及促进活性氧自由基(reactive oxygen species,ROS)生成的机制.彗星实验发现:PM10 2.5,PM2.5 0.1和PM0.1对2种细胞均有显著的基因毒性,并呈剂量-效应关系;细和超细颗粒造成的DNA损伤显著高于粗颗粒;HUVEC细胞的DNA损伤程度大于A549细胞;PM2.5 0.1和PM0.1可诱导2种细胞内ROS水平显著升高,而PM10 2.5不能.因此细胞内DNA损伤可能与ROS生成有一定联系.

Ambient particulate matter has become one of the most harmful pollutants in the air. The geno- toxic effects and generationmechanism of reactive oxygen species （ROS） were studied in A549 and HUVEC cells treated with three sizes of ambient particles, PM10-2.5, PM2.5-on and PM0.1. The results from comet assay show that PM10-2.5, PM2.5-0.1 and PMon caused significant DNA damages in both A549 and HUVEC cells in a dose-dependent manner. In both types of cells, the DNA damage caused by PM2.5-0.1 and PM0.1 are significantly higher than that caused by PMlo-2.5, and PMs cause higher DNA damage in HUVEC cells than that in A549 cells. PM2.5-0.1 and PMo.1 can significantly increase the intracellular ROS level, but PM10-2.5 cannot. DNA damage in cells induced by particles may be related to the generation of ROS, which deserves further investigations.