MSAP技术及其在玉米遗传育种上的研究进展

Methylation-Sensitive Amplified Polymorphism and Its Research Progress on Maize Genetic Breeding

DNA甲基化是生物基因组最常见,也是最重要的表观遗传修饰形式之一,随着对DNA甲基化研究的不断深入,由AFLP技术发展而来,基于PCR检测的DNA甲基化敏感扩增多态性(MSAP)技术,凭借其应用广泛,操作简便等优点成为检测DNA甲基化水平的有力工具。目前已被广泛应用于种质资源鉴定、品种改良、基因表达调控等许多领域。本文对MSAP技术的原理、操作流程及其在玉米遗传育种研究中的各项研究进展进行了综述,并对该技术今后的应用前景进行了展望。

DNA methylation is one of the most important and common modifications in epigenetics.With progress of the studies on DNA methylation,methylation-sensitive amplified polymorphism（MSAP）,which is developed from AFLP technology and basing on the PCR technology,has been a effective means for detecting DNA methylation levels because of its wide range of applications and simple of operation.It has been widely applied to many fields currently,such as germplasm identification,variety improvement and regulation of gene expression.In this paper,the principles and operational processes of MSAP technology and applications of MSAP in maize genetics and breeding were summarized.Also the future of applications prospects of MSAP technology was carried out.