丙型肝炎病毒体外培养技术的新进展

The New Development of Culture Technique In vitro of Hepatitis C Virus

丙型肝炎病毒(hepatitis C virus,HCV)是引起丙型肝炎的病原体,全球约1.7亿人感染HCV.高效体外细胞培养体系的缺乏,严重影响了HCV病毒生命周期的阐明以及抗病毒药物和预防性疫苗的研发.2005年Wakita T建立了JFH1/Huh7真正的HCV体外培养体系,为HCV研究提供了必要的研究条件.其后,研究者为提高病毒复制效率,简便病毒检测方法,在病毒株的选择替换、重组病毒构建、宿主细胞的改造筛选及病毒培养操作技术改进方面做了大量工作,取得了较好进展,为HCV的病毒学研究、药物筛选以及疫苗的研发提供了更有效的技术途径.

Hepatitis C virus （HCV）, the major pathogen of hepatitis, had infected approximately 170 million people throughout the world. Due to the absence of efficient in vitro cell culture system, the life cycle and functional mechanism of HCV are still poorly understood, and the development of vaccine and antiviral drugs are seriously hampered. Since the establishment of JFH1/Huh7 HCV culture system by Wakita T in 2005, it has provided a useful tool for HCV research. Since then, in order to improve the efficiency of virus replica- tion and simplified methods of detecting virus, a large amount of achievements have been made in selective exchange of HCV virus strains, construction of chimera virus, optimization of the host cell and improvement of the virus culture technique. The related achievements may provide the useful tool for the study on HCV virological characters and the development of anti-virus treatment and vaccine.