胡桃楸叶片POD同工酶表达特点的研究

Study on Expression Characteristics of Peroxidase Isozymes in the Leaves of Juglans mandshurica Maxim

为探究胡桃楸生长发育特点，促进其引种栽培和食药用开发，利用聚丙烯酰胺凝胶电泳检测其5～9月整个生长期叶片中POD同工酶的表达情况，通过迁移率绘制表达图谱并进行分析。结果表明：5～9月胡桃楸叶片整个生长期共14种POD同工酶表达，具有阶段特异性；其中8月叶片中同工酶表达数量多且表达量高，为叶片成熟期；叶片中POD同工酶的表达能够反映生长期生理代谢活动的变化。

For further development of J uglans rnandshurica Maxim on edible and medicinal,as well as introduction and cultivation,the growth characteristics of Juglans mandshurica Maxim were discussed by using polyacrylamide gel electrophoresis to detect the expression of POD isozymes in leaves from May to September,and the mobility expression map was drawn and analyzed. The results showed that a total of 14 kinds of POD isozymes were expressed in the whole leaf growing period from May to September with stage specificity; Isozyme expression in August was the highest, this time was maturation stage of Juglans mandshurica Maxim; Expression of POD isozymes in leaves reflected the change of physiological metabolic activity in growth period.