甘蓝型油菜BnKCS10基因的克隆和功能分析

Cloning and functional characterization of BnKCS10 gene from Brassica napus

为探讨编码β-酮酰-辅酶A合成酶(β-ketoacyl coa synthase,KCS)基因家族成员之一的KCS10基因功能,本文通过基因组步移和RACE技术,从甘蓝型油菜中双9号中克隆了BnKCS10基因。甘蓝型油菜BnKCS10基因编码区长2 344bp,含有两个内含子,长度分别为527bp和167bp。BnKCS10基因在花蕾中的表达量最高,其次是柱头和受精后30d的角果,说明在生殖器官中的表达量明显高于营养器官。转BnKCS10基因的酵母能够正常合成预期的蛋白,但没有超长链脂肪酸的合成。

KCS10 gene is one of the members of the β-ketoacyl-CoA synthase(β-ketoacyl coa synthase,KCS) gene family.To understand the function of KCS10 gene,genome walking and RACE techniques were used to clone BnKCS10 gene from B.napus cv.Zhongshuang 9.The BnKCS10 gene has a 2 344bp-coding region containing 2 introns spanning 527bp and 167bp respectively.BnKCS10 gene expressed at significantly higher levels in flower buds,and also in stigma and 30d developing seeds than in the vegetative organs.The yeast cells tranformed with BnKCS10 gene produced KCS10 protein normally,but no synthesis of very long chain fatty acids was detected.