交联剂对脱细胞膀胱基质生物力学性能的影响

Effects of different crosslinking agents on biomechanical properties of acellular bladder matrix

目的研究不同交联剂对猪脱细胞膀胱基质的组织结构影响,并比较其生物力学性能,为盆底修复替代材料的选择提供依据。方法采用表面活性剂+酶消化法去除新鲜猪膀胱的细胞成分,将脱细胞膀胱基质随机分为3组,A组经0.25%戊二醛交联,B组经0.625%京尼平交联,C组未交联。对各组材料进行HE染色,观察纤维的变化情况。使用生物力学性能测试系统检测抗拉强度、断裂伸长率、弹性模量,并进行统计学分析。结果京尼平交联脱细胞膀胱基质后呈深蓝色,保持了天然组织构架的完整,纤维更加致密。戊二醛交联脱细胞膀胱基质后呈浅黄色,纤维排列紊乱且有断裂。新鲜猪膀胱经上述三种方法处理后,其弹性模量增大、断裂伸长率减小,而其中京尼平交联处理的脱细胞膀胱基质力学性能与新鲜膀胱组织更为相近。结论京尼平交联的脱细胞膀胱基质组织结构的形态佳,同时较大限度地保留膀胱组织的力学性能,可能是较理想的盆底重建材料。

Objective To compare the effects of different crosslinking agents on structures and biomechanical properties of porcine acellular bladder matrix. This could provide the basic selection of ideal biomaterials for the reconstruction of female pelvis. Methods Cellular components of fresh porcine bladder were removed by detergent-enzymatic method. They were randomly divided into three groups,and group A was crosslinked with 0.25% glutaraldehyde,group B was crosslinked with 0.625% genipin,group C wasn’t crosslinked. Then all samples were examined the morphology with hematoxylin and eosin staining,and biomechanical tests were also performed and evaluated the biomechanical properties by statistical analysis. Results The genipin-crosslinked acellular bladder matrix was dark blue,with very well preserved collagen fiber. The glutaraldehyde-crosslinked acellular bladder matrix was light yellow,with disordered and fracture fiber. The elastic modulus of the three groups increased and break elongation decreased compared with the fresh bladder tissue,and the genipin-crosslinked acellular bladder matrix was similar with the fresh bladder tissue. Conclusions The genipin-crosslinked acellular bladder matrix possesses good organization structure form,and effectively preserves bladder tissue mechanical properties. Therefore the genipin-crosslinked acellular bladder matrix may be ideal material for pelvic floor reconstruction.