Extraction of Geniposide and Its Application in Anesthesiology 被引量：1

Extraction of Geniposide and Its Application in Anesthesiology

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摘要 We tried to develop an efficient strategy to isolate geniposide from Gardenia fruit and use it to treat chronic pain. Eight extraction solvents and three extraction methods were respectively compared with each other for the efficiency of geniposide extraction from Gardenia fruit. Extraction with 80% ethanol under reflux was found to give the highest yield of geniposide. Orthogonal experiment was conducted to optimize the reflux conditions and the optimal conditions are a mass（of Gardeniajasminoides Ellis powder） to volume（of solvent, 80% ethanol） ratio of 1：7 and reflux twice for 60 rain each. The geniposide obtained by the reflux method was tested on the rats with chronic constriction injury（CCI） for pain relief. The results show that geniposide can enhance pain threshold and relieve pain via repressing tumor necrosis factor-a（TNF-a） expression in either ipsilateral or contralateral dorsal root gan- glion（DRG） cells. These results suggest that geniposide treatement may be an effective clinical method to relieve pain based on the mechanism of molecular biology at DRG due to nerve injury. We tried to develop an efficient strategy to isolate geniposide from Gardenia fruit and use it to treat chronic pain. Eight extraction solvents and three extraction methods were respectively compared with each other for the efficiency of geniposide extraction from Gardenia fruit. Extraction with 80% ethanol under reflux was found to give the highest yield of geniposide. Orthogonal experiment was conducted to optimize the reflux conditions and the optimal conditions are a mass（of Gardeniajasminoides Ellis powder） to volume（of solvent, 80% ethanol） ratio of 1：7 and reflux twice for 60 rain each. The geniposide obtained by the reflux method was tested on the rats with chronic constriction injury（CCI） for pain relief. The results show that geniposide can enhance pain threshold and relieve pain via repressing tumor necrosis factor-a（TNF-a） expression in either ipsilateral or contralateral dorsal root gan- glion（DRG） cells. These results suggest that geniposide treatement may be an effective clinical method to relieve pain based on the mechanism of molecular biology at DRG due to nerve injury.

作者 XU Dan DONG Yu-xiang FENG Wei GE Hong-yan LI You-tian JIANG Yang WANG Li-jie SONG Da-qian

机构地区 The First Hospital of Jilin University China-Japan Union Hospital of Jilin University College of Chemistry

出处《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2013年第4期724-729,共6页 高等学校化学研究（英文版）

基金 Supported by the Natural Science Foundation of Jilin Province, China（No.20125059）.

关键词 GENIPOSIDE Reflux method Orthogonal experiment Neuropathic pain Geniposide Reflux method Orthogonal experiment Neuropathic pain

分类号 Q786 [生物学—分子生物学] TQ461 [化学工程—制药化工]

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