摘要
目的:探讨缺氧缺血性脑病(HIE)患儿血清中S-100B蛋白和神经元特异性烯醇化酶(NSE)的水平变化、相关性的临床意义及其与NBNA评分间的相关性和临床意义。方法:HIE组足月HIE患儿35例,轻度10例,中度15例,重度10例;对照组正常足月新生儿25例。采用酶联免疫吸附法(ELISA)检测两组1 d和3 d血清中的S-100B蛋白和NSE的水平。结果:HIE新生儿1 d血清S-100B蛋白及NSE水平显著高于正常对照组,病情越重,血清S-100B蛋白水平及NSE越高(F=149.4,214.8,P=0.00);出生后3 d内正常对照组血清S-100B蛋白、NSE水平无明显变化(t=0.956,0.858,P>0.05),轻度HIE新生儿出生后1 d较高,3 d后明显下降,差异有显著意义(t=6.78,4.586,P﹤0.001),中度HIE新生儿出生后1 d较高,3 d后有所下降,差异有统计学意义(t=2.255,2.515,P﹤0.05),重度HIE生后3 d内血清S-100B蛋白及NSE均维持在高水平,差异无统计学意义(t=1.015,0.789,P>0.05);生后第3 d,HIE组血清S-100B蛋白和NSE呈正相关(r=0.923,P=0.000),S-100B蛋白和NSE与NBNA评分均呈负相关(r=-0.656,P=0.000;r=-0.479,P=0.001)。结论:中重度HIE患儿血清S-100B蛋白和NSE的浓度明显高于对照组,与其临床分度基本一致。监测HIE患儿血清中S-100B蛋白和NSE的水平,对疾病的早期诊断、病情程度的判断、治疗效果的评价具有一定的临床价值。
Objective: To study the changes o f serum concentrations of S-100B protein and Neuron-specific enolase (NSE) in newborns with hypoxie-ischemic encephalopathy( HIE), the correlation between S-100B protein and NSE, and between S-100B protein and NSE and neonatal behavioral neurological assessment (NBNA), and clinical significance. Methods :Thirty-five term newborns with HIE were divided into three groups, mild HIE group (n = 10) , moderate HIE group (n = 15 ) and severe HIE group (n = 10). Twenty-five term normal newboms were served as control group. Serum concentrations of S-IOOB protein and NSE were measured by enzyme-linked immunosorbent assay (ELISA). Results:One day after birth, the level of S-100B protein and NSE in HIE neonate were much higher than normal neonate. The more se- rious condition, the higher was for S-IOOB protein and NSE ( F = 149.4,214.8, P = 0.00 ). The control group for blood serum S-100B protein and NSE level had no obvious change(t =0.956,0.858 ,P 〉0.05) for first 3 days after born,dl af- ter born was slightly higher for mild HIE group, and drop dramatically after 3 days, the difference had great significance ( t = 6.78,4. 586 ,P 〈 0. 001 ) ;dl after born was slightly higher for moderate HIE group, and drop after 3 days ,the differ- ence had statistics significance( t = 2. 255,2. 515 ,P 〈 0.05) ; the blood serum S-100B protein and NSE level maintained in high level for severe HIE group within 3 days after born, the difference had no statistics significance (t = 1. 015, 0. 789 ,P 〉 0.05 ), and there was a significant positive correlation between S-100B and NSE ( r = 0. 923, P = 0.000 ) , there was a significant negative correlation between S-100B and NBNA (r = -0. 656,P = O. 000) , and there was a nega- tive correlation between NSE and NBNA( r = -0. 479 ,P=0. 001 ) . Conclusion:The levels of S-100B protein and NSE in moderate and severe HIE infants were approximately higher than those in control group, and they were identical with the severity of HIE ,so they may be useful markers for the diagnosis of HIE. The measurement of S-100B protein and NSE will be very important to the early diagnosis and judgment of therapeutic efficacy.
出处
《赣南医学院学报》
2013年第4期520-522,525,共4页
JOURNAL OF GANNAN MEDICAL UNIVERSITY