多个顺式作用元件调控t-PAcDNA乳腺定位表达

Multiple Cis acting Elements Regulating t PA cDNA Expression in Mammary Gland

由牛 β-酪蛋白 ( β-Casein)启动子与大鼠乳清酸蛋白 ( WAP)启动子融合及串联构建成 2个组织纤溶酶原激活剂 ( t-PA) c DNA乳腺定位表达载体 p SVL-βΔWAP-PA和 p SVL-WAPβ-PA。将这 2种表达质粒分别注入怀孕家兔乳腺导管中 ,溶圈试验结果显示 ,2种质粒均能在家兔乳腺中表达 ,且以分娩后第 5天的乳汁中表达水平最高 ,分别为 70 0 μg/ L和 50 0 μg/ L。本试验为进一步研究 t-PA基因乳腺定位表达调控和建立

Two specific expression pasmids, pSVL β ΔWAP PA and pSVL WAPβ PA, containing t PA cDNA gene,were constructed by fusing with cattle β Casein promoter, respectively.The two plasmids were injected directly into pregnant rabbit mammary gland duct. The result showed that both plasmids could express t PA in rabbit milk.The expression level was 700 μg/L and 500 μg/L on day 5, respectively. It provides the useful information for study the regulation of specific expression in mammary gland and the establishment of t PA mammary gland bioreactor.