骨髓间充质干细胞移植对心肌梗死大鼠损伤心肌的修复效果

Repair of Infarcted Myocardium by Bone Marrow Mesenchymal Stem Cell Transplantation in Rats

目的探讨骨髓间充质干细胞(BMSCs)移植对心肌梗死大鼠损伤心肌的修复效果,为进一步提高BMSCs移植治疗心肌梗死的疗效提供实验依据。方法将22只大鼠参照Olivette方法制作心肌梗死模型,共16只进入实验终点,随机分为2组:对照组(C组,n=8)梗死区注射PBS;骨髓间充质干细胞移植组(M组,n=8)梗死区注射BMSCs。术后喂养4周,行心脏彩超,取心脏组织,荧光显微镜观察BMSCs的分布、分化及梗死区毛细血管密度。结果标记CM-Dil和cTnT免疫组织化学染色双重阳性的心肌样细胞在梗死区分布。与C组相比,M组大鼠左室收缩末期内径(LVEDd)、左室舒张末期内径(LVEDs)、射血分数(EF)、左室短轴缩短率(FS)均有明显改善(P<0.05),毛细血管密度明显增加(P<0.05)。结论 BMSCs可在心肌梗死的大鼠心脏分化为心肌样细胞,提高梗死区毛细血管密度,改善心功能。

Objective To observe the efficacy of the transplantation of bone marrow mesenchymal stem cells（BMSCs） in the repair of injured myocardium in rats with myocardial infarction,and to provide experimental evidence for improving the treatment of myocardial infarction.Methods Myocardial infarction was induced according to Olivette ＇ s method in 22rats.The 16rats with myocardial infarction were randomly divided into two groups,with 8rats in each group.Rats in transplantation group were injected with BMSCs in the infarct region.Rats in control group were given PBS injection.The heart function was assessed by echocardiography and heart tissue was taken 4weeks after operation.The distribution and differentiation of BMSCs and capillary density in infarct area were observed under fluorescence microscope.Results The cardiac-like cells positive for both CM-Dil and cTnT were observed in infarct area.Compared with control group,left ventricular end-systolic diameter,left ventricular end-diastolic diameter,ejection fraction and left ventricular fractional shortening were improved and capillary density was increased in transplantation group（P0.05）.Conclusion BMSCs can differentiate into cardiac-like cells,promote angiogenesis and improve cardiac function in the infarcted heart.