附植前小鼠胚胎上H-Y抗原表达的研究

H Y Antigen Expression in Preimplantive Mouse Embryos

用 8～ 1 2周龄 BALB/c公鼠采取皮肤移植和脾细胞腹腔注射 2种方式免疫同日龄纯系 BALB/c母鼠 ,制备 H-Y抗血清。经精子微量细胞毒性试验 ,筛选的抗血清用于胚胎体外培养 ,以鉴定附植前小鼠胚胎上 H-Y抗原的表达。选用 8cell-早期囊胚进行体外培养 ,分别于 5～ 6、1 8和 2 4 h观察胚胎发育情况。 5～ 6h后 ,H-Y抗血清培养的胚胎退化率高达 4 0 %,且发育迟缓 ,与自然性比例差异不明显 ( P>0 .0 5)。正常血清培养的胚胎 2 4 h左右 。

H Y antisera were produced in 8 12 weeks old of BALB/c female mice by skin grafts and repeated intraperitioneal injections of syngenetic male spleen cells,and detected by sperm microcytotoxicity test Then the chosen antisera were used in preimplantational embryos culture to detect the H Y expression 8cell early blastula embryos were used in vitro culture After 5 6,18 and 24 h of culture,the embryos were classified as either affected or unaffected After 5 6 h of culture,percentage of affected embryos was 40%,there was no significant difference from the normal sex ratio(1∶1,P>0 05) Embryos cultured in normal serum could grow to morula or blastula