一个新的水稻矮秆突变体的遗传分析和基因定位

Genetic Analysis and Fine-Mapping of a Novel Dwarf Mutant in Rice

新的水稻矮秆基因的发掘,对深入研究植物株高的调控途径及株型育种有非常重要的作用。本研究报道了从日本特早熟粳稻品种Kitaake的组织培养后代获得的一个矮秆突变体dm,该突变体植株细小,紧凑,机械强度降低,结实率下降,子粒变窄,千粒重降低等。利用分离群体中的矮秆株,最终将目标基因定位在第4染色体长臂末端InDel标记EL-72和L-1之间,物理距离为168 kb的区间内,该区间内无已报道的水稻矮秆基因,该基因可能是一个尚未被克隆的新的株高决定基因。

Screening of dwarf mutants plays an important role in understanding of the molecular mechanism in rice plant architecture. We report here the identification and characterization of a novel rice dwarf mutant from tissue culture progeny of a Japonica variety Kitaake,dm. The mutant shows a pleiotropic phenotype,including dwarf,frag- ile stem,low fertility, and decreased 1000-grain weight. By using F2 and its following segregated populations, we nar- rowe down dm gene into a 168 kb region on the long arm of chromosome 4. Our results indicate that DM represents a new locus responsible for rice plant architecture.