3个烤烟品系对TMV抗性的遗传规律分析

Inheritance for Resistance to TMV in Three Tobacco Lines

为更好地利用抗TMV烤烟种质资源,提高烤烟抗TMV育种效率,对烤烟品系CV87、FC8、抗88的TMV抗性遗传规律及抗性来源进行了研究。利用抗病烤烟品系CV87、FC8、抗88分别与感病品种云烟87、中烟100配置杂交组合,构建F1、F2群体,并利用TMV-C菌株进行抗性鉴定;同时,设计N基因引物对参试烤烟品种(系)的基因组DNA进行PCR扩增。经抗性鉴定,CV87、FC8、抗88及F1群体对TMV免疫,云烟87、中烟100感TMV,卡方(χ2)检验证明F2群体抗感分离比为3∶1,符合显性单基因遗传;PCR结果表明,抗病品系CV87、FC8、抗88基因组内存在N基因序列,感病品种云烟87、中烟100基因组内未发现。本研究表明,CV87、FC8、抗88烤烟品系的TMV抗性来源于N基因。

For effective use of tobacco germplasm resources resistant to TMV, and improve resistance breeding efficiency against TMV,the inheritance for TMV resistance and resistance gene in three flue-cured tobacco lines, CV87,FC8 and Kang88,were studied. CV87, FC8 and Kang88 were crossed with YunYan87 or ZhongYanl00, re- spectively,and F1 plants were self-pollinated to produce F2 populations, then the TMV resistance of CV87, FCS, Kang88 and there F1. F2 populations were identified by inoculating TMV-C strain;In addition,N gene primers were used to detect N gene sequence in genomic DNA of CV87, FC8 ,Kang88 ,YunYan87 and ZhongYanl00. Resistance identification showed that CV87, FC8, Kang88 and F1 group were immune to TMV, YunYan87 and ZhongYanl00 were susceptible to TMV,and Chi-square （X2） tests proved that the separation ratio of F2 groups were 3：1 ,in ac- cordance with the dominant single gene inheritance;PCR results found that N gene was present in resistant lines, CV87 ,FC8, Kang88 ,did not exist in susceptible materials,YunYan87 and ZhongYanl00. The results indicated that the TMV resistance of CV87,FC8 and Kang 88 were controlled by single dominant gene N.