烟草种质不同群体量遗传完整性的SSR研究

Analysis on Tobacco Genetic Integrity of Different Populations by SSR Markers

以普通烟草种质红花大金元、豌口红土烟、白花黑烟、云烟87以及野生种Nicotiana alata为试验材料,利用SSR分子标记技术结合构建DNA混合基因池的方法对种质不同群体量的遗传完整性进行研究。结果表明,960对引物对红花大金元、豌口红土烟、白花黑烟以及云烟87进行全基因组扫描,在前3份种质中未筛选到多态性引物,而在云烟87中筛选出3对多态性引物。3对多态性引物在云烟87的80个单株中扩增出6条特异性条带,将群体量降为10株时仍能检测到6条特异性条带,因此普通烟草种质繁殖更新群体等于或大于10株便能代表群体的遗传完整性。野生种N.alata从608对引物中筛选出11对多态性引物,扩增出44条DNA条带,其中多态性DNA片段有19条,并对不同的群体量进行遗传多样性参数的比较,得出大于20株的群体能代表野生种的遗传完整性。

Nicotiana tabacum germplasm Honghuadajinyuan, Wankouhongtuyan, Baihuaheiyan, Yunyan 87 and wild tabacco germplasm Nicotiana alata were used as materials in this study. The genetic integrity of different popu- lations was investigated by SSR with constructing mixed DNA pool. The results showed that none of polymorphic SSR primers were screened from 960 SSR primer pairs of Honghuadajinyuan,Wankouhongtuyan and Baihuaheiyan, but 3 polymorphic SSR primers of Yunyan 87 were detected. 6 polymorphic alleles were amplified from 80 plants, and they could still be achieved when the population was reduced to 10 plants. So the population greater than or e- qual to 10 plants can represent the genetic integrity of N. tabacum germplasm. 11 pairs of primers were selected from the 608 pairs to analyze N. alata,and 44 alleles were amplified from the 80 plants,and the polymorphic alleles were 19. According to the comparison of genetic diversity parameters,a sample Of no less than 20 plants should be repre- sent the genetic integrity of the population.