黄褐斑肝郁证小鼠模型建立的研究进展

Research Progress of ChloasmaModel Preparation of Liver Qi Stagnation Pattern/Syndrome in Mice

建立具有中医特点能够进行中医证候分型的实验动物模型是制约中药新药开发过程中药理学研究的瓶颈问题。黄褐斑是一种常见的影响面部美容的疾病,如何建立符合中医辨证论治用药特点的黄褐斑肝郁证模型是研发治疗该病证新药的关键。文章简述了近年来关于黄褐斑小鼠模型建立方法,从中尝试寻求建立黄褐斑肝郁证小鼠模型的科学方法,为临床上开发有效治疗黄褐斑的中药新药,提供更为系统、科学的实验动物模型。分析表明黄体酮注射+紫外线照射+束缚方法是建立该病证小鼠模型比较可靠的方法。增加检测皮肤组织中酪氨酸、丙二醛含量、超氧化物歧化酶活度更能全面评价黄褐斑改善生化指标。借助前期课题组在肝郁证动物模型研究基础,增加旷场、糖水偏好、强迫游泳等行为学方法,则更能客观评价肝郁证行为学指标。

It is the bottleneck problem to set up the experimental animal model for pattern/syndrome differentiation in accordance with the characteristics of traditional Chinese medicine（TCM） to restrict the pharmacological research in the development of new drug of TCM.Chloasma is the common cosmetic disease.In the research and development of new drug of the disease,the point is to build the model of chloasma differentiated as liver qi stagnation in compliance with the pattern / syndrome differentiation and determination of treatment in TCM.The paper introduced briefly the method of model preparation of chloasma differentiated as liver qi stagnation in mice in recent years and attempted to find the scientific method for the setup of the model of chloasma differentiated as liver qi stagnation in mice so as to provide more systematic and scientific experimental animal model for the clinical research and development of new drug of TCM for the treatment of chloasma.The analysis indicated that progesterone injection ＋ ultraviolet irradiation ＋ bound method was the relatively acceptable approach to the preparation of mice model.The detection of tyrosine and malondialdehyde expressions as well as superoxide dismutases activity in skin tissue could evaluatemuch more completely the changes of biochemical indexes in chloasma.On the basis of the previous research on the animal model of chloasma differentiated as liver qi stagnation,the behavior methods such as open-field test,syrup preference and forced swimming were supplemented,which assessedmore objectively the behavior indexes in liver qi stagnation.