鸡传染性支气管炎病毒浙江分离株RFLP基因分型

Restriction fragment length polymorphism analysis of Zhejiang isolates of avian infectious bronchitis virus

为了查清浙江省目前流行的鸡传染性支气管炎病毒 (IBV)的基因型 ,我们收集了 5个不同疫区的 IBV(肾病变型 J、W、X、N;腺胃病变型 ZJ971)和参考株 H12 0 ,分别对其 S1基因 c DNA进行 Hae 、Sty 、Pst 、Ksp6 32 的 RFLP分析 .结果表明 IBV- J、W、X、N株的 RFLP基因类型相同 ,但与古老的肾病变型代表株 (即 T株、Gray株、Holte株 )、国内外报道的肾病变型毒株及呼吸型 H12 0 株均不同 ,是一种新的 RFLP基因类型 ;IBV- ZJ971株的基因型与 H12 0

WT5”BZ]In order to make clear the genotypes of infectious bronchitis virus (IBV)isolated in Zhejiang province. cDNA of the S1 gene of five IBV isolates from different regions, including four nephropathogenic isolates J,X,W,N and one proventriculus isolates ZJ 971 ,and one reference strain H 120 , were amplified by reverse transcription polymerase chain reaction and digested with restriction enzymes HaeⅢ, StyⅠ, PstⅠ, and Ksp632Ⅰ.The patterns of restriction fragment length polymorphism analysis showed that isolate ZJ 971 was the same genotype as strain H 120 . IBV isolates.J,W,Xand N have identical genotype. However, their RFLP patterns were different from other nephropathogenic IBV isolate reported in the literature, such as the classic nephropathogenic strains T,Gray and Holte, and the respiratory strain H 120 . The results indicated that isolates J,X,W,N were putative variant strains of IBV.