猪下丘脑促生长激素释放激素(GHRH)基因克隆及序列分析

Cloning and sequence of GHRH gene of porcine hypothalamus

从杜长大杂交猪的下丘脑中提取基因组 RNA,根据报道的猪 GHRH c DNA序列设计引物 1和引物 2 ,用反转录 -聚合酶链式反应 (RT- PCR)进行 c DNA扩增 ,获得一条 132 bp的片段 ,克隆于 p GEM-T Easy载体后进行序列分析表明 ,该片段为 GHRH基因的 c DNA,它由 132个核苷酸组成 ,编码 4 4个氨基酸组成的多肽 ,即 GHRH.本研究扩增得到的基因片段与报道的猪

WT5”BZ]Porcine ds RNA was extracted from hypothalamus of finishing pig(Duroc×Yorkshire×Landrace). A pair or primers was designed according to the GHRH gene sequence reported previously. A cDNA fragment in length 132 bp was synthesized and amplified by RT PCR. The PCR product was cloned into pGEM T Easy vector and then sequenced.It was demonstrated that this fragment was GHRH gene cDNA.It has 132nts in length,which encodes a polypeptide containing 44 amino acids. Homogenious analysis showed that it was consistent with the reported porcine GHRH gene sequence.