摘要
用RT PCR技术从人胎盘组织内成功地扩增全长肝细胞生长因子 (HGF)cDNA基因 (2 184bp) ,并将其克隆至pGEM T载体 ,经限制性核酸内切酶NdeⅠ ,Bg1Ⅱ ,HindⅢ ,BamHⅠ和XhoⅠ的酶谱分析和DNA测序分析证实。再将其亚克隆至逆病毒载体pLNL XHC 。
The full length of HGF cDNA gene(2?184?bp) was amplified successfully from human placental tissue using RT PCR technique, and then cloned into pGEM T vector, which was identified and confirmed by restriction endonuclease mapping using NdeⅠ, Bg1Ⅱ, HindⅢ, BamHⅠ and XhoⅠ, as well as DNA sequencing. The above clone of HGF cDNA gene was successfully subcloned into the retrovirus vector(pLNL XHC), which may be used for further studies of gene expression and gene therapy. [
出处
《湖南医科大学学报》
CSCD
2000年第5期425-427,共3页
Bulletin of Hunan Medical University
基金
国家自然科学基金!资助 ( 3 970 0 0 5 0 )
