大鼠胚胎小脑提取液对体外培养小脑神经细胞的作用

Effect of cerebellum extracts from embryonic rat on cultured cerebellar neurons

目的 观察不同胎龄大鼠小脑提取液 (cerebellum extracts,CE)对体外培养小脑神经细胞的影响。方法 制备不同蛋白浓度的 14、16、18、2 0 d胎鼠 (E14、E16、E18、E2 0 )及新生 1d鼠 (P1) CE,加入原代培养的E18胎鼠小脑神经细胞中 ,2 4h后以微量快速自动比色法检测细胞活性。结果 实验组各胎龄 CE均能促进培养小脑神经元的存活 ,并有蛋白浓度依赖关系 ,各胎龄都有各自不同的最适浓度 ,E16和 E18CE显示了更强的神经营养活性 ,与对照组及其他实验组相比差异有极显著性 (P<0 .0 0 1)。结论 CE对培养小脑细胞具有高度神经营养活性。

Objective The effects of cerebellum extracts (CE) from differentembryonic ages on cultured rat cerebellar neurons were studied.Method CE from 1 4d,1 6d,1 8d,2 0 d embryonic rats (E1 4～ 2 0 ) and P1 newborn rat were made into different concentrations on protein,then were added to cultured cerebellar neurons of E1 8rats.The neuronal survival was determined by the autocolorimetric assay.Results The data showed that CE of all experimental ages could promote the survival of cerebellar neurons and there was a concentration- dependent relation on protein;each age had its respective optimun protein concentration,E1 6and E1 8CE showed more prominent neurotrophic activities,making a significant difference (P <0 .0 0 1 ) cempared with the control and other experimental groups. Conclusion The rat cerebellum extract (CE) has a powerful trophotropic effect on the cultured cerebellar neurons.