激发光诱导奶牛血液的荧光光谱特性研究

Fluorescence Spectrum Characteristics of Cow Blood Induced by Exciting Light

为研究300～500nm激发光诱导奶牛血液的荧光光谱特性,以正常奶牛和异常奶牛(患乳腺炎)的血液样品为研究对象,利用荧光分光光度计对样品进行了荧光光谱试验。结果显示,当激发光波长为340～380nm时,血液样品产生了较强的荧光且具有较强的规律性。荧光强度较强的位置为469、605、642nm,并且样品的荧光光谱呈现出宽谱带特征,在468～491nm范围内,正常奶牛样品的荧光强度明显大于异常奶牛的荧光强度。分析表明,患乳腺病的奶牛其血液中的黄素腺嘌呤二核苷酸浓度发生变化,导致468～520nm范围内荧光发生变化;血液中的卟啉分子发生变化,导致676～800nm范围内荧光发生变化。

In this paper,the fluorescence characteristics of healthy cow＇s blood and suffering （mammitis） cow＇s blood samples were studied by the fluorescence spectrophotometer.The results showed that when the wavelength range of excitation light was 340 nm to 380 nm,there were strong fluorescence intensity peaks in blood sample with regularity.The strong fluorescence peaks located at the 469,605,642 nm.The fluorescence peak of healthy cow＇s blood sample was stronger than that of the abnormal cow＇s blood sample,especially in the wavelength range of 468nm to 491 nm.By the analysis of the test results,when the cow suffers mammitis disease,the flavin concentration of adenine dinucleotide in the blood changes,which leads to the changes of fluorescence in the wavelength range of 468 nm to 520 nm.Meanwhile,the porphyrin molecules in the blood have also changed that leads to the changes of fluorescence in the wavelength range of 676 nm to 800 nm.