摘要
通过对武威市凉州区人参果感病植株所表现出来的花叶、皱缩、植株矮小等症状观察,初步判断可能感染了马铃薯M病毒(PVM)。结合分子生物学手段,提取感病人参果叶片总RNA,采用RT-PCR方法扩增到一段长300 bp的基因序列,连接至PMD19-T载体并测序。测序结果同GeneBank中PVM CP基因序列[GI:361071296]同源性达到了98.97%,说明成功克隆到了PVM基因片段,即供试人参果确实感染了PVM病毒。
The mosaic, wrinkled, small plant and other symptoms of susceptible plants of Liangzhou ginseng fruit were observed, the auditor should make a pre-judgment that may be infected with potato virus M (PVM). Experiments combined with molecular biology methods and extract leaves total RNA of the patient feeling of ginseng fruit, it was amplified by RT-PCR method to a long 300 bp gene sequences and connected to PMD19-T vector and sequenced. The Sequencing results showed that homology reached 98.97%with the GeneBank PVM CP gene sequence [GI: 361071296], it can explain gene fragment was cloned into the PVM successful, that the test does ginseng fruit infected with PVM virus.
出处
《甘肃农业科技》
2013年第8期18-20,共3页
Gansu Agricultural Science and Technology
基金
甘肃省科技支撑计划"用融合基因dsRNA获得抗病性人参果种质资源的研究"(1011NKCA077)部分内容
甘肃省农牧厅农业生物技术专项"导入甜蛋白基因创造甜味人参果种质研究"(GNSW-2012-22)部分内容
关键词
人参果
PVM病毒
克隆
鉴定
Ginseng fruit
PVM virus
Cloning
Identification