藏獒16个STR基因座的遗传多态性

Genetic Polymorphisms of 16 STR Loci in Tibetan Mastiff

目的通过对449头藏獒的16个STR基因座遗传多态性进行研究,建立藏獒的基因多态性数据库。方法选用犬荧光标记16个STR复合扩增试剂盒进行PCR扩增,对扩增产物进行检测及统计学分析。结果 449头藏獒的16个STR基因座累积个体识别率为0.999 999 999 999 999,累积非父排除率为0.999 997 795,除FH2010(等位基因数10)、PEZ21(等位基因数12)、PEZ05(等位基因数13)外,其余STR基因座的等位基因数均在15个以上;16个STR基因座的杂合度均>0.5,多态信息含量均>0.7。结论 16个犬STR基因座在藏獒中具有较高的个体识别能力,可用于藏獒的个体识别和亲权鉴定。通过本研究获得的各种数据,可用于建立藏獒的DNA多态性数据库。

Objective To explore the genetic polymorphisms of 16 STR loci from 449 Tibetan Mastiffs in order to set up gene polymorphism database of Tibetan Mastiff.Methods The PCR amplification was performed using the 16 STR loci fluorescent multiple amplification kit for dog.The amplified products were detected and statistically analyzed.Results In the 16 STR loci from 449 Tibetan Mastiffs,CDP was 0.999 999 999 999 999 and CEP was 0.999 997 795.Except FH2010（10 alleles）,PEZ21（12 alleles）,and PEZ05（13 alleles）,the other STR loci had more than 15 alleles.In the 16 STR loci,H was 0.5 and PIC was 0.7.Conclusion The 16 STR loci have high polymorphism to be suitable for individual identification and paternity testing of Tibetan Mastiff.The data obtained through this study can be used to establish DNA polymorphism database of Tibetan Mastiff.