姜黄素对人微血管内皮细胞增殖、迁移、成管以及凋亡的影响

Effect of Curcumin on Human Microvascular Endothelial Cell Proliferation, Migration, Tube Formation and Apoptosis in Vitro

【目的】研究姜黄素对人微血管内皮细胞(HMVEC-L)增殖、迁移、成管以及凋亡的影响。【方法】将姜黄素分为0、20、40、80μmol/L 4个实验组,分别用非放射性细胞增殖检测(MTS)法、划痕实验、成管实验以及Hoechst 33258与TUNEL双重荧光法检测姜黄素对HMVEC-L增殖、迁移、成管以及凋亡的影响。【结果】MTS结果显示:从姜黄素与HMVEC-L共同培养的第1天开始,20～80μmol/L浓度组均可抑制HMVEC-L增殖,与对照组(0μmol/L)比较差异有统计学意义(P<0.05或P<0.01),尤以80μmol/L组抑制HMVEC-L增殖能力最显著(P<0.05或P<0.01)。划痕实验结果显示:20～80μmol/L姜黄素浓度组HMVEC-L迁移率均显著降低,各组HMVEC-L迁移率比较差异有统计学意义(P<0.05),其中尤以80μmol/L组HMVEC-L迁移率最低。成管实验结果显示:20～80μmol/L姜黄素浓度组HMVEC-L成管长度均显著降低,各组HMVEC-L成管长度比较差异有统计学意义(P<0.05),其中尤以80μmol/L组HMVEC-L成管长度最小。Hoechst 33258与TUNEL双重荧光染色结果显示:在姜黄素与HMVEC-L共同培养过程中HMVEC-L发生了凋亡。【结论】姜黄素可通过抑制HMVEC-L增殖、迁移、管样形成并诱导其发生凋亡,从而有效抑制肿瘤血管形成。

Objective To study the effect of curcumin on the proliferation, migration, tube formation and apoptosis of human lung microvascular endothelial cells （HMVEC-L） in vitro. Methods The HMVEC-L were co-cultured with different concentrations of curcumin （0, 20, 40 and 80 μmol/L） , and then HMVEC-L proliferation, migration, tube formation and apoptosis were measured by non-radioactive MTS/PES assay, scratch adhesion test, Matrigel assay and Hoechst33258/TUNEL fluorescence staining, respectively. Results The results of non-radioactive MTS/PES assay showed that HMVEC-L proliferation was suppressed by 20 - 80μmol/L of curcumin, in particular by 80 μmol/L of eurcumin, from the first day of co-cuhure （P〈0.05 or P〈0.01 compared with the control group at 0 μmol/L） The results of scratch adhesion test showed that HMVEC-L migration rate was decreased by 20- 80 μmol/L of curcumin （P〈0.05）, and the rate was the lowest in curcumin group of 80 μmol/L. The co-culture of curcumin at 20 - 80 μmol/L also reduced the length of formative tube of HMVEC-L, and the effect of 80μmol/L of curcumin was the strongest （P〈0.05） Matrigel assay and Hoechst33258 /TUNEL fluorescence staining showed that migration and tube formation was suppressed by curcumin treatment, and the apoptosis of HMVEC-L was presented after the co-culture with curcumin. Conclusion Curcumin may play the role of tumor angiogenesis suppression through the inhibition of HMVEC-L proliferation, migration, tube formation and apoptosis.