冠心苏合丸对乳鼠心肌细胞缺血再灌注损伤的作用

Effect of Guanxin Suhe Pills on Neonatal Rat Cardiomyocytes with Ischemia-reperfusion Injury

【目的】研究冠心苏合丸对缺血再灌注(ischemia reperfusion injury,IRI)损伤心肌细胞的保护作用,探讨其作用机制。【方法】培养乳鼠心肌细胞,建立缺血再灌注损伤细胞模型,采用血清药理学方法,药物血清组给予体积分数10%的含药血清处理。观察计数心肌细胞搏动频率;测定各组培养液中乳酸脱氢酶(LDH)、肌酸激酶(CK)、超氧化物歧化酶(SOD)、丙二醛(MDA)的含量及细胞半胱天冬蛋白酶(Caspase-3)活性;采用流式细胞仪测定细胞凋亡率。【结果】冠心苏合丸可显著提高缺血再灌注损伤心肌细胞的搏动频率,降低细胞培养液中的LDH、CK及MDA含量,提高SOD的含量,降低心肌细胞Caspase-3活性及细胞凋亡率(均P<0.05或P<0.01)。【结论】冠心苏合丸可能通过抑制Caspase-3活性及细胞凋亡发挥对心肌缺血再灌注损伤的保护作用。

Objective To study the protective effect of Guanxitt Suhe Pills on cardiomyocytes suffering ischemia- reperfusion injury, and to investigate its mechanism. Methods The cultured neonatal rat cardiomyocytes were separated by trypsin digestion. Cardiomyocyte model of ischemia-reperfusion injury was established by ischemia for 3 hours and reoxygenation for one hour subsequently. By using the sero-pharmacological method, the drug serum treatment group was treated with 10% serum containing Gttanxin Suhe Pills. After treatment, pulsefrequency of myocardial cells was counted, the contents of lactate dehydrogenase （LDH） , creatine kinase （CK）, superoxide dismutase（SOD）, and malondialdehyde（MDA） in the culture medium as well as cardiomyocyte caspase-3 activity were detected by colorimetry, and apoptotic rate was determined by flow cytometry. Results Guanxin Suhe Pills showed obvious effect on increasing pulse frequency of injured cardiomyocytes, reducing the LDH, CK, MDA levels in culture medium, increasing the content of SOD, and decreasing cardiomyocyte caspase-3 activity and apoptotic rate （P〈0.05 or P〈0.01 ）. Conclusion Through decreasing cardiomyocyte caspase-3 activity and apoptotic rate, Guanxin Suhe Pills can protect cardiomyocytes from ischemia-reperfusion injury .