乌骨藤总苷抑制肝癌细胞增殖和AFP分泌

Effects of Total Glycosides in Glaucescent Fissistigma Root on the Proliferation of Hepatocarcinoma Cells and Secretion of AFP

目的研究乌骨藤总苷对肝癌细胞增殖的作用及其机制。方法采用四甲基偶氮唑盐比色法(MTT比色法)或移植瘤小鼠模型,观察不同浓度乌骨藤总苷对人肝癌Bel-7404、HepG2细胞和小鼠肝癌Hepl-6细胞生长的影响;研究乌骨藤总苷抑制HepG2细胞生长与调控甲胎蛋白AFP分泌的关系。结果乌骨藤总苷对人肝癌Bel-7404、HepG2细胞和小鼠肝癌Hepl-6细胞生长有显著的抑制作用,能显著降低AFP的分泌。结论乌骨藤总苷抑制肝癌细胞增殖其机制可能与抑制AFP分泌有关。

Objective To investigate the effects of total glycosides in glaucescent fissistigma root on the proliferation of hepatocarcinoma cells. Methods MTF assay and hepatoma model in mice were used to observe the proliferation of hepatoma cells impacted by total glycosides in glaucescent fissistigma root. Expression of AFP in HepG2 cells was analyzed by radio immunoassay. Results Total glycosides in glaucescent fissistigma root can significantly inhibit the proliferation of human and murine hepatoma cancer cells, and significantly reduce the secretion of AFP in HepG2 cells. Conclusion Total glycosides in glaucescent fissistigma root can inhibit the proliferation of liver cancer cells , which mechanism may be related to inhibit the secretion of AFP.