二烯丙基二硫抑制HL-60细胞增殖及对c-myc基因表达调控

Proliferation Inhibition and c-myc Expression Down-Regulated by Diallyl Disulfide in HL-60 Cells

目的研究二烯丙基二硫(DADS)对人髓系白血病细胞株HL-60细胞增殖及对c-myc基因表达的影响。方法采用MTT和细胞记数法分析DADS对细胞生长的影响、蛋白印迹法检测细胞内c-myc蛋白表达水平。结果 DADS能抑制HL-60细胞增殖,并呈剂量和时间依赖性(P<0.05),DADS(20μmol/L)作用12 h后能下调c-myc蛋白的表达水平。结论 DADS抑制HL-60细胞增殖,其机制可能与下调c-myc表达水平密切相关。

Objective To investigate the effects of DADS on proliferation inhibition and on c-myc protein expression in human myeloid leukemia cell line HL-60 cells. Methods HL-60 cells were exposed to DADS at different dosages. Growth inhibition was detected by MTr assay and cell growth. The expression of c-myc was measured by Western blotting. Results After treatment with DADS, the growth of HL-60 cells were suppressed in a concentration-dependent and time- dependent manner（ P 〈0.05 ）. Incubation of HL-60 cells with DADS（20 i.Lmol/L）for 12 h could decrease the expression of c-myc. Conclusion DADS could inhibit HL-60 cells proliferation, its mechanism may be closely related with adjusting expression level of the c-myc.