摘要
目的探讨钩端螺旋体(钩体)外膜蛋白Loa22免疫血清与不同血清型钩体间的交叉免疫反应作用,为筛选钩体候选疫苗分子提供依据。方法制备钩体重组Loa22蛋白、LipL32蛋白豚鼠免疫血清,以LipL32免疫血清为阳性对照,未免疫豚鼠为阴性对照,ELISA测定与我国常见的各血清型钩体株的交叉反应的Loa22蛋白免疫血清效价。结果与钩体犬群犬型56603株、致热群致热型56605株、澳洲群澳洲型56607株、波摩那群波摩那型56608株、流感伤寒群临海型56609株、七日群七日热型56610株及明尼群明尼型56655株反应的钩体Loa22蛋白免疫血清效价分别为1∶81 920、1∶20 480、1∶40 960、1∶40 960、1∶40 960、1∶10 240和1∶10 240。结论Loa22蛋白免疫血清与不同血清型钩体有良好的交叉免疫反应性。
Objective To investigate the crossimmunoreactivity between antisera against Leptospiral outer mem brane protein loa22 and various serotypic leptospira, providing a basis for screening Leptospiral candidate vaccine mole cules. Methods The antisera were obtained from guinea pigs immunized with recombinant Loa22 or LipL32. With anti sera against LipL32 as positive control and normal sera as negative control, ELISA was performed to determine the titers of antisera against loa22 which reacted with various common serotypic leptospira in China. Results The titers of antisera a gainst Loa22 which reacted with various pathogenic L. interrogans strains 56603,56605,56607,56608,56609,56610 and 56655 were 1：81 920,1：20480,1：40960,1：40960,1：40960,1：10240 and 1：10 240,respectively. Conclusion The antisera against Leptospiral outer membrane protein Loa22 show good crossimmunoreactivity with various serotypic Lcpto spira.
出处
《中南医学科学杂志》
CAS
2013年第4期350-352,共3页
Medical Science Journal of Central South China
基金
遵义医药高等专科学校资助项目(遵医专科合[2001]210号)
