猪、牛和兔粪中致病性大肠杆菌LEE毒力岛的检测分析

Detection and Analysis of Pathogenic Escherichia coli and LEE PAI in Porcine,Bovine and Rabbit Feces

畜禽粪便中含有大量微生物,在发生传染性疾病时,粪便常常会成为重要的传染源。通过从种猪场、奶牛场和种兔场粪便中分离鉴定致病性大肠杆菌,并对分离到的大肠杆菌进行LEE毒力岛上eaeA基因的PCR检测,鉴定出含有LEE毒力岛大肠杆菌,调查携带LEE毒力岛的大肠杆菌在猪、牛和兔粪便中的存在情况。实验结果表明,猪、牛、兔粪便样品中携带LEE毒力岛的大肠杆菌检出率分别为12.9%、14.0%、22.5%。eaeA阳性的大肠杆菌应是导致仔猪、10日龄以内的初生犊牛和兔场断奶后幼兔发生腹泻症的主要细菌性病原。LEE毒力岛的检测能够作为大肠杆菌的诊断和流行分子病学调查的主要手段。

Many different kinds of germs were detected in animal feces which often act as important infectious sources when diseases occurred.The research was carried on to isolate Escherichia coli from the feces of the pig breeding farm,cattle farm and rabbit breeding farm,and then a PCR detection experiment of the eaeA gene on the LEE PAI was conducted to study the existence situation of the Escherichia coli owned LEE PAI in the animals.The experiment results showed that detection rate of Escherichia coli in pig,cattle and rabbit were 12.9%,14.0%,and 22.5% respectively.The conclusions were that positive Escherichia coli carried with the eaeA gene was the main cause of diarrhea in animals.LEE pathogenicoty island could be a main method for EPEC molecular diagnosis and epidemiological investigation.