髓过氧化物酶基因G-463A多态性与脑梗死易感性的研究

Relation between myeloperoxidase G-463A gene polymorphisms and susceptibility to cerebral infarction

目的探讨髓过氧化物酶（MPO）基因G-463A位点多态性与颈动脉斑块患者易发脑梗死的相关性。方法用二维超声测量97例颈动脉斑块患者和68名健康者内,中膜厚度（IMT）及颈动脉斑块指数（PI），限制性片段长度多态性,聚合酶链反应（RFLP-PCR）技术检测MPO基因G-463A多态性。结果MP0-463位点G／A多态性有3种基因型（GG，GA，AA），2组间基因型分布和等位基因频率分布差异有统计学意义（P〈0．05）。具有GG基因型者发生脑梗死的危险性较具有AA基因型者显著增高[χ2=6．925，P=0．008，相对危险度（OR）=4．030，95％可信区间（CI）：1．465—11．236]；杂合子GA发生脑梗死的危险性较纯合子AA仍有升高趋势（χ2=3．43，P=0．006，OR=2．748，95％CI：0．952—7．800）；将GA和GG基因型合并计算，发现携带至少1个等位基因G者其患脑梗死的风险仍显著增高（χ2=6．20，P=-0．013，OR=3．488，95％CI：1．302～9．468）。结论MPO基因G-463A位点多态性与颈动脉斑块程度关系明显。MPO基因G-463A位点多态性与颈动脉斑块患者易发脑梗死的关系明显。

Objective To investigate the relation between myeloperoxidase gene G-463A polymorphisms and the susceptibility to ischemic stroke in patients with carotid arteriosclerosis. Methods Two-dimensional ultrasonog- raphy was employed tO determine the intima-media thickness （IMT） of the common carotid artery and plaque forma- tion, and polymerase chain reaction restriction fragment length polymorphism technology （PCR-RFIP） was used to de- tect MPO genotype MPO-463 polymorphisms in 97 patients with carotid arteriosclerosis and 68 healthy subjects. Re- stilts There was a statistically significant difference （both P〈0.05） in the distribution and frequency of alleles of the three G/A genotypes of MPO-643 （GG, GA and AA）. Compared with AA genotype, the GG genotype was associated with an increased risk of infarction （odds ratio： 4.030, 95% confidence interval： 1.465-11.236, P=0.008）. The het- erozygote GA genotype had, compared with the homozygote AA genotype, an increased risk of infarction （odds ratio： 2.748, 95% confidence interval： 0.952-7.800, P=0.006）. Patients with at least one G allele had, following the combi- nation of GA and GG genotypes, an increased risk of infarction （odds ratio： 3.488, 95% confidence interval： 1.302- 9.468, P=0.013）. Conclusion MPO gene G-463A polymorphism is significantly linked to the degree of carotid atherosclerosis and correlates with the episodes of stroke.