摘要
目的研究大鼠血浆中红景天苷的口服及静脉给药的药代动力学，并进行生物利用度的评估。方法将健康SD大鼠16只随机分组，采用静脉注射和灌胃2种不同的方式给药（48mg／kg），采血测定血浆内红景天苷的血药浓度。结果主要药物代谢动力学参数：灌胃：t1／2为（0．64±0．11）h，ρmax为（13．9±2．9）μg／mL，AUC0～12和AUG0-∞分别为（16±4）μg·h^-1·mL^-1和（16±4）μg·h^-1·mL^-1；静脉注射：t1／2为（1．16±0．44）h，AUC0～12和AUC0-∞分别为（38±11）μg·h^-1·mL^-1和（39±12）μg·h^-1·mL^-1；绝对生物利用度为41．9％。结论红景天苷吸收迅速，生物利用度良好。
Objective To study the pharmacokineties of salidroside in rat plasma, and evaluate the absolute bioavailability. Methods Eighteen healthy SD rats were randomly divided into two groups, and a dose of intrave- nous and oral administration（48 mg/kg） were delivered, respectively. The plasma concentrations of salidroside were determined by LC-MS/MS method. Results The main pharmacokinetic parameters were as follows： ig： t1/2 （0.64±0.11）h,ρmax（13.9±2.9）μg/mL, AUC0～12 AUG0-∞ （16±4）μg·h^-1·mL^-1 （16±4）μg·h^-1·mL^-1; AUC0～12 AUG0-∞（16±4）μg·h^-1·mL^-1,（16±4）μg·h^-1·mL^-1, AUC0-∞ （38±11）μg·h^-1·mL^-1,（39±12）μg·h^-1·mL^-1, respectively. Conclusion This simple and sensitive LC-MS/MS method was successfully applied to determination of drug concentration and pharmacokinetic studies of Salidroside. The salidroside could be absorbed fast after oral administration with a good bioavailability.
基金
沈阳市科技局科学技术计划项目(130406)
