摘要
目的研究通宣理肺丸对CYP2D6酶活性的影响，探讨通宣理肺丸的代谢途径，为临床指导合理用药提供依据。方法筛选30名健康志愿者作为受试者。以右美沙芬作为CYP2D6的探针药，研究服用通宣理肺丸对人体内代谢酶CYP2D6活性的影响。采用高效液相色谱法-质谱（HPLC—MS／MS）法测定探针药及其代谢产物的血药浓度。连续服用14d通宣理肺丸。以曲线下面积（AUC0～12）为指标，评价CYP2D6代谢酶的活性。结果受试者用药前CYP2D6代谢酶活性为6．1±1．5，服用通宣理肺丸后代谢酶活性为6．5±1.7，差异无统计学意义。结论服用通宣理肺丸对CYP2D6代谢酶活性没有明显影响。
Objective To investigate the effect of Tongxuan Lifei pill on CYP2D6 and to provide foundation for clinical drug using. Methods Thirty volunteers were divided into two groups according to CYP2D6 gene type with dextromethorphan used as probe drug of CYP2D6. The effect of Tongxuan Lifei pill on the activity of CYP2D6 was investigated. Genetic classification was fulfilled by pyrosequencing method, and the blood concentration of probe drug and its metabolic product was determined by HPLC-MS/MS. The volunteers took Tongxuan Lifei pill for two weeks and AUC0-12 was used as the standard of the activity of CYP2D6. Results The activity value was 6.1± 1.5 before administration of drug and 6.5 ± 1.7 after drug using. The activity of CYP2D6 was decreased after drug using, t-test was used for comparison, there existed no remarkable difference between the data. Conclusion Tongxuan Lifei pill has no obvious affection on the activity of CYP2D6.
