Kiss-1和E-cadherin在声门上型喉鳞状细胞癌中的表达及临床意义

The expression of Kiss - 1 and E - cadherin in squamous cell carcinoma of the supraglottic larynx and their clinical significance

目的观察Kiss-1和E钙粘蛋白（E-cadherin）在人声门上喉鳞状细胞癌组织中的表达及两者间的相互关系，初步探讨Kiss-1、E-cadherin在人喉鳞状细胞癌发病机制中的作用及临床意义，为喉鳞状细胞癌的预后判断提供理论依据。方法应用免疫组化sP法检测78例声门上喉鳞状细胞癌及78例正常声门上喉组织的Kiss-1及E-cadherin蛋白的表达情况，评估声门上喉鳞状细胞癌中Kiss-1及E-cadherin蛋白表达与临床及病理因素的关系，并分析二者之间相关性。结果Kiss-1和E-cadherin蛋白阳性表达主要位于细胞膜。78例声门上喉鳞状细胞癌组织中Kiss-1和E-cadherin蛋白的阳性表达率分别为32．1％和42．3％；78例喉正常组织中Kiss-1和E-cadherin蛋白的阳性表达率分别为62．8％和83．3％。两种抑癌基因在声门上喉鳞状细胞癌组织和正常喉组织中的表达有统计学差异（P〈0．01），Kiss-1和E-cadherin在喉鳞状细胞癌颈淋巴结转移、临床分期及分化程度具有统计学差异（P〈0．05），Kiss-1和E-cadherin在喉鳞状细胞癌的年龄、性别上无统计学差异（P〉0．05）；KiSS-1和E-cadherin之间的表达呈正相关，两者间差异具有统计学意义（P〈0．01）。结论Kiss-1和E-cadher-in蛋白在声门上喉鳞状细胞癌组织中的表达明显缺失，提示Kiss-1和E-cadherin可能在喉鳞状细胞癌的发生发展中起重要作用；Kiss-1和E-cadhefin蛋白表达与声门上喉鳞状细胞癌的淋巴结转移、临床分期及分化程度有关，与患者的性别及年龄无关，Kiss-1和E-cadherin的表达存在相关性。

Objective To investigate the expressions of Kiss - 1 and E - eadherin in laryngeal squamous cell carcinoma, as well as to approach the role and correlation of Kiss - 1 and E - cadherin. Methods Kiss - 1 and E - cadherin expressions were detected in 78 laryngeal squamous cell carcinoma and 78 normal laryngeal tis- sue samples by immunohistochemical method to analyze the correlation between the protein expression rate and clinical and pathological characters and study the correlation between the expression of Kiss - 1 and E - cadherin in squamous cell carcinoma of the supraglottic larynx. Results Expression of every gene was significantly differ- ent between laryngeal squamous cell carcinoma and normal laryngeal tissues（P 〈 0. 001 ）. The negative expression rate of Kiss - 1 and E - cadherin was significantly associated with cervical lymph node metastasis and clinical stage （ P 〈 0.001 ）, but not associated with sex and age of the patients （P 〉 0.05）. The negative expression of Kiss - 1 is closely related with the negative expression of E - cadherin（ P 〈0.001 ）. Conclusion The expressions of Kiss - 1 and E - cadherin in case and control group has a significant difference. Kiss - 1 loss of expression as well as E - cadherin may be a biological maker in squamous cell carcinoma of the supraglottic larynx. The levels of Kiss - 1 and E - cadherin were useful molecular markers for evaluating malignancy degree and lymph node metas- tasis of in laryngeal squamous cell carcinoma, and may play important roles in inhibiting the invasion and metastasis