支原体对细胞培养污染的研究概况

Introduction on Mycoplasma Contamination in Cell Culture

支原体是自然界中存在的最小、最简单的原核生物,大小介于细菌和病毒之间,可通过滤菌器,对许多抗生素有抗性,是细胞培养污染中一种常见的微生物。被支原体污染后的细胞培养物,引起细胞形态学和功能的改变,导致用细胞基质制备的生物制品报废,且很难清除。一旦发生污染,就意味着试验的失败,造成巨大的人力、物力、财力的浪费。从支原体对细胞培养物污染后造成的危害及对支原体污染的预防、检测、清除等的研究进展进行论述,为细胞支原体污染的防控提供依据和手段。

Mycoplasma is the smallest and simplest prokaryotes in nature,which size is intermediate betw- wen virus and bacteria. It can pass though bacteria-filtration device, and is resistant to lots of antibiotics. Mycoplasma is a frequently occurring microorganism. Mycoplasrna contamination normally results in cell morphological and function change. As Mycoplasma is to hard to be clearified from cell culture,Mycoplas- ma contamination will lead the scrap of biological products based on cell culture, and huge waste of man- power, material and financial resources. The review introduced the harmful effect of Mycoplasma contami- nation, and how to prevent, detect,and eliminate which would offer the scientific basis and means to pre- vent and contol Mycoplasma contamination in cell cultures.