摘要
目的 观察羊膜对其基质面培养的 YAC- 1细胞增殖的影响。方法 将 YAC- 1细胞接种于羊膜基质面 ,用 MTT自动比色法分别于接种后 1、3、7d检测羊膜对 YAC- 1细胞增殖的影响。结果 接种后 1、3、7d,羊膜均对 YAC- 1细胞有明显的促增殖作用 (P<0 .0 1)。结论 羊膜有促进肿瘤细胞增殖的作用 。
Objective To investigate the effect of amniotic membrane on the proliferation of YAC 1 in vitro. Methods The YAC 1 cells were cultured with amniotic membrane and their proliferation were observed by MTT automated colorimetry on the lst day, the 3rd day and the 7th day respectively.Results Amniotic membrane could significantly enhance the proliferation of YAC 1 on the lst day, the 3rd day and the 7th day respectively after having been seeded ( P <0 01).Conclusion Amniotic membrane can enhance the proliferation of tumor cells (YAC 1), which should be paid attention to during amniotic membrane transplantation for treating ocular surface lesion caused by epibulbar tumors.
出处
《眼科新进展》
CAS
2000年第5期317-318,共2页
Recent Advances in Ophthalmology
基金
上海市科学技术发展基金重大资助项目!(编号:9941190 08)
关键词
羊膜
淋巴瘤细胞
细胞增殖
眼表面肿瘤
amniotic membrane
YAC 1 cell
cell proliferation
epibulbar tumor
