大孔吸附树脂对虎杖中白藜芦醇的分离纯化研究

Purification of Reveratrol from Polygonum cuspidatum by Macroporous Adsorption Resin

旨在筛选出分离纯化虎杖中白藜芦醇的最佳大孔吸附树脂以及工艺条件。静态吸附与解吸、动态吸附与解吸相结合的方法,以树脂的最大吸附量、解吸率为考察指标,确定最佳的纯化工艺。H103树脂对白藜芦醇有较好的吸附与解吸效果,其最佳的工艺条件为粗提液中白藜芦醇的质量浓度为0.72mg/mL、上样流速2BV/h,吸附饱和后先用4BV的蒸馏水进行洗涤,然后用8BV、75%的乙醇溶液以1.5BV/h的流速进行洗脱,白藜芦醇的含量可由纯化前的12.8%提升至53.5%。应用H103树脂对虎杖中的白藜芦醇进行纯化,其工艺稳定可行,具有吸附快、解吸容易、解吸液安全低毒且回收容易的特点,具有较高的应用价值。

Various macroporous resins with different properties were employed to purify the resveratrol from the roots of Polygonum cuspidatum. Static and dynamic adsorption and desorption experiments were conducted, meanwhile, the adsorption and desorption rates of resveratrol were concerned to test out the prominent macroporous resin and optimal processing parameters. H103 was subsequently selected as the best macroporous resin. The optimal processing parameters were obtained as follows： the crude extract containing resveratrol of 0.72 mg/mL was loaded at a flow rate of 2 BV/h, the H103 column was washed with 4 BV of distilled water for removing the impurities, and elution with 8 BV of 75% ethanol was at a flow rate of 1.5 BV/h. As a result, the content of purified resveratrol was increased from 12.8% to 53.5%. The method with H103 as the optimal macroporous resin undergoing with the modified purification process is a simple, safe and inexpensive technology, and has a promising potential of purifying resveratrol from the roots of Polygonum cuspidatum.