摘要
目的 建立一种快速测定体外培养表皮细胞生长增殖的方法.方法 利用MTT只被活细胞还原成紫色颗粒的特性,通过比色分析测定吸光度值,用于检测细胞的数量与活性;并与3H-TdR掺入法进行比较.结果 表皮细胞量在0.125~2.0×105细胞/孔时与所测定的吸光度值呈一良好线性关系(r=0.9689).用MTT法测定人表皮细胞在二种不同培养基中生长增殖与3H-TdR掺入法所测结果意义一致.结论 MTT法用于测定表皮细胞的生长增殖简便、迅速。
Objective This study was to devolop a new method that can rapidly measure the growth and survival of human keratinocyte HK. Method The colorimetric assay of MTT for living cells was utilized to determine the growth and Survival of human keratinocyte because MTT was only modified to a dark blue formazan product by living cell.The optical densityODvalues were read on a scanning multiwell spectrophotometerELISA reader. Results There was well linear relation between the optical density values and the quantity of HK at 0.125~2.0×105cells/well.The meaning of MTT method was similar to that of 3H-TdR for measuring proliferation of HK in two different kind of serum-free medium. Conclusion MTT method is convenientrapid and reliable for measuring the proliferation of HK it is worth spreading
出处
《江西医药》
CAS
2000年第5期263-264,共2页
Jiangxi Medical Journal