MTT法快速测定人表皮细胞的生长增殖

Rapid MTT method assay for the proliferation of human keratinocyte

目的 建立一种快速测定体外培养表皮细胞生长增殖的方法.方法 利用MTT只被活细胞还原成紫色颗粒的特性,通过比色分析测定吸光度值,用于检测细胞的数量与活性；并与3H－TdR掺入法进行比较.结果 表皮细胞量在0．125～2．0×105细胞／孔时与所测定的吸光度值呈一良好线性关系(r＝0．9689).用MTT法测定人表皮细胞在二种不同培养基中生长增殖与3H－TdR掺入法所测结果意义一致.结论 MTT法用于测定表皮细胞的生长增殖简便、迅速。

Objective This study was to devolop a new method that can rapidly measure the growth and survival of human keratinocyte HK． Method The colorimetric assay of MTT for living cells was utilized to determine the growth and Survival of human keratinocyte because MTT was only modified to a dark blue formazan product by living cell．The optical densityODvalues were read on a scanning multiwell spectrophotometerELISA reader． Results There was well linear relation between the optical density values and the quantity of HK at 0．125～2．0×105cells／well．The meaning of MTT method was similar to that of 3H－TdR for measuring proliferation of HK in two different kind of serum－free medium． Conclusion MTT method is convenientrapid and reliable for measuring the proliferation of HK it is worth spreading